The Limitations of Bradford Assay Method
The Bradford Assay is a broadly used protein quantification technique developed in 1976 by Marion M. Bradford. It’s fast, easy, and price efficient; nevertheless, there are some limitations that needs to be considered when utilizing this method. Firstly, the Bradford Assay depends on the presence of a dye-binding protein, which might trigger errors in protein quantification if the dye binds to non-protein parts within the pattern. This generally is a specific subject when quantifying proteins in complicated samples resembling serum or cell lysates. (Liang, et al., 2016). Secondly, the Bradford Assay is delicate to the adjustments in pH, ionic energy and temperature of the pattern, which might trigger inaccuracies within the outcomes. The pattern ought to at all times be buffered at a impartial pH, and cautious consideration needs to be paid to the temperature of the pattern when performing the assay (Chen, et al., 2021).
Thirdly, the Bradford Assay is just not appropriate for figuring out the focus of enormous proteins, because the dye can solely bind to proteins with a molecular weight of as much as 50 kDa (Sakthivel, et al., 2016). Cont…
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