Name the compound used to visualize DNA bands after electrophoresis. Also, describe any special precautions or handling and explain why such may be needed. Ethidium Bromide: A Compound Used to Visualize DNA Bands After Electrophoresis

Ethidium Bromide: A Compound Used to Visualize DNA Bands After Electrophoresis

Ethidium bromide (EtBr) is a strong compound used to visualise DNA bands after electrophoresis. Electrophoresis is a separation course of whereby molecules are moved by way of a gel matrix below the affect of an electrical area. EtBr acts as an intercalating agent, which binds to the DNA strands and emits a fluorescent sign when uncovered to an ultraviolet (UV) mild supply. This makes it attainable to detect bands of DNA on an agarose gel. On account of its carcinogenic properties, particular precautions have to be taken when dealing with Ethidium bromide. It is strongly recommended that protecting gloves and eye safety be worn when dealing with EtBr. Moreover, it needs to be dealt with in a well-ventilated surroundings, because the vapors might be hazardous if inhaled. All containers needs to be labeled with the suitable warning notices (Chen et al., 2019). It is usually essential to keep away from contact with pores and skin and clothes, in addition to keep away from ingestion of the compound.
The carcinogenic properties of EtBr have been identified because the Nineteen Seventies (Chen et al., 2016), and its use has been discouraged in favor of safer options. Cont….